Title : Characterization of isolated strains of microorganisms from mineral, mountain, and spring waters from France, Italy, England, South Korea, Japan, the Netherlands, Austria, Spain, Singapore, Germany, Switzerland, Greece, Turkey, Dubai, and Bulgaria.
Abstract:
The aim of the present work is to isolate, identify and investigate the species of bacteria from healing, mountain and spring waters in the area of Paris, France, Rome, Italy, London, England, Busan, South Korea, Tokyo, Japan, Amsterdam, Holland, Vienna and in Bulgaria. 127 bacterial strains were isolated and their colonial and morphological characteristics were determined and the study strains were identified. The data show that the isolated 9 strains from the hypothermal healing spring waters in the Paris area, France were identified as N. Valcheva - Lisinibacillus pakistanensis, Serratia marcescens (four strains), Staphylococcus hominis, Staphylococcus haemoliticus, Bacillus pumilus and Bacillus cereus. The isolated strains from thermal healing springs in Saturnia, Tuscany region and Fontana di Trevi in Rome, Italy were identified as N. Valcheva - Tiobacillus Ferrooxidans, Staphylococcus cohnii, from a hypothermal spring in the London area as N. Valcheva - Aeromonas caviae, Pseudomonas chlororaphis, from a mountain spring in the area of Busan, South Korea, was identified as N. Valcheva - Bacillus safensis, Staphylococcus cohnii, Pseudomonas chlororaphis, Staphylococcus cohnii. From a mountain geothermal spring in the area of Tokyo, Japan, strains were identified as N. Valcheva - Bacillus safensis, Phellodendron chinense, Aeromonas caviae, Pseudomonas japonica. The strains identified in the Amsterdam, Netherlands area were identified as N.Valcheva&A.Atanasova - Aeromonas viridans, N.Valcheva&V.P.Panteleev - P. japonica, N.Valcheva&V.V. Panteleev - Aeromonas salmonicida, N.Valcheva&A.V. Panteleev - Aeromonas caviae, N.Valcheva&L.A.V. Panteleeva - Phellodendrom chinense. Sulfur bacteria of the species N. Valcheva - Chlorobium chlorochromatii were identified from the healing springs in the region of Baden and Vienna, Austria. The strains from Bulgaria were identified from 100 hypothermal, thermal and common springs from 11 regions and belong to the following 14 genera: N. Valcheva - Bacillus, Brevibacillus, Geobacillus, Aeromonas, Klebsiella, Pseudomonas, Staphylococcus, Stenotrophomonas, Serratia, Nocardia, Trichosporon, Azoarens , Lisinbacillus, Rhodococci. From the hypothermal salt source in Barcelona, in the area to the Mediterranean Sea, 29 species of organisms were identified N.Valcheva §St.Mihailov - Pseudomonas shengduensis, N.Valcheva&V.Marinova-Bacillus cereus (3 strains), N.Valcheva&I.D.Valchev - Shevanella algae (3 strains), N.Valcheva&D.I.Valchev - Acinetobacter quillouiae, N. Valcheva&N.I.Valcheva - Pseudomonas pseudoalcaligenes, N.Valcheva&I.I.Valcheva - Pseudomonas korensis, N.Valcheva&P.N.Valcheva - Delftia acidovorans (2 strains), N.Valcheva&M.P.Pantelev - Citrobacter frendii, N. Valcheva&M.I.Panteleeva - Pseudomonas rhodesiae, N.Valcheva&D.Eschkenazy - Pseudomonas Chloraphis, N. Valcheva&H.Eschkenazy - Pseudomonas korecusis, N.Valcheva -M.Eschkenazy - Pseudomonas chlororaphis, N. Valcheva&G.Dimov - Strenotrophomonas sp., N.Valcheva&M.Naneva - Preudomonas rhodesiae, N.Valcheva& N.Nanev - Enterobacter Ludwigii, N. Valcheva&St.Stratiev - Aeromonas caviae (2 strains), N.Valcheva&P. Panteleev - Aeromonas mediae, N.Valcheva& E.Todorovska - Providencia retgerri, N.Valcheva - A.Stoyanova -Myroides odoratiminus, N.Valcheva&R.Moneva - Alcaligenes faecalis. N.Valcheva - Bacillus safensia is the only test that shows a significant reduction in the number of cells by 50% compared to cells in lung cancer A549. Concentration from 100 μg /mL. Metabolomic profiling of crude extracts from N.Valcheva - Bacillus safensis, isolated from a source in Tokyo and a source in Busan, South Korea, near the spring of South China Sea, reveals the presence of known antibacterial and/or anticancer agents such as licorin, angustin, krinamidin, Vaccinol and poulin.N. Valcheva - Acinetobacter guillouiae(born in Barcelona) degradates plasma. With probiotic action from N.Valcheva - Bacillus subtilis and N. Valcheva - Bacillus methylotrophius(Bulgaria). N.Valcheva&I.I.Valcheva - Pseudomonas korensis is used for vaccination. The identified strains from a source in the Singapore area are: N.Valcheva- Aeromonas caviae, Klebsiella oxytoca, Kurthia gibsoni, Roatella ornithinolitica, Kurthia gibsonii, Pseudomonas aeruginosa, Citrobacter cloacae, Klebsiella pneumoniae, Alcaligens fecalis, Aeromonas veronii, Serratia marcescens, Enterobacter hormchei, Enterobacter cloacae, Citrobacter frendii, Debaromyces hansenii, Pseudomonas putida, Pseudomonas sp.. The identified strains from a spring in the Berlin, Germany area are: N.Valcheva- Klebsiella, pneumoniae, N.Valcheva&A. Dechev, N.Valcheva&T. Bazaitova Serratia liquafaciens,N.Valcheva - Aerococcus oxytoca, Buttiauxella gaviniae, Debaromyces hansenii. All isolated strains have enzyme, anti-fungal and antibacterial activity. Remediation is used to extract valuable metals or remove metals that are harmful to organisms.All strains have different biological activity. The difference in the type of strains identified can be explained by the influence of the composition of the water, the type of soil and rocks through which it passes, the temperature, the pH of the environment and the different geographical location.
